ABSTRACT
Aim: The aim of this study is to demonstrate the relation between the expression of liver alpha-amylase and obesity
Background: Alpha-amylase catalyses the hydrolysis of 1, 4-alpha-glucosidic linkages in polysaccharides and has three main subtypes, including: salivary, pancreatic, and hepatic. Hepatic alpha-amylase is involved in glycogen metabolism, and has a role in obesity and its management. In this study, we aimed to analyze the expression of liver alpha-amylase in overweight and obese mouse
Material and methods: In this study, NMRI male mice were randomly divided into two groups. The sample group [obese] took a high-fat and carbohydrate diet, while the control group [normal] took a laboratory pellet chow for eight weeks. During this period, their weight was measured. After eight weeks, liver hepatocytes were isolated using an enzymatic digestion method. Immunocytochemistry [ICC] and flow cytometry analysis were performed to measure alpha amylase protein expression in mouse liver hepatocyte cells
Results: A significant difference in the body weight was observed between the two groups [p<0.05]. The qualitative protein expression of liver alpha-amylase was found to be higher in the obese group in both tests [immunocytochemistry and flow cytometry]. Animals from the test group presented higher alpha-amylase expression, which suggests that this hepatic protein may constitute a potential indicator of susceptibility for fat tissue accumulation and obesity. The present data demonstrates an increased expression of liver amylase in obese mice
Conclusion: These results suggest that liver amylase secretion might be useful for predicting susceptibility to obesity induced by consumption of a high-fat and carbohydrate diet